Curr Opin Chem Biol 28:174180 Dubowchik GM, Firestone RA, Padilla L, Willner D, Hofstead SJ, Mosure K, Knipe JO, Lasch SJ, Trail PA (2002) Cathepsin B-labile dipeptide linkers for lysosomal release of doxorubicin from internalizing immunoconjugates: model studies of enzymatic drug release and antigen-specific in vitro anticancer activity
The most common reconstitution mistakes involve rushing this step
Since imine bonds hydrolyze under acidic conditions, the gatekeeper molecules such as PEG, polyglycerol, peptide, and carbohydrate could be severed from the surface at low pH thus opening the pores and releasing the loaded guest molecules 131,132,133
Since most peptides were not approved, it was illegal to sell them as medicines in some countries, including the U.S
J Diabetes Res 2018