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Disulfide cross-linked protein represents a significant fraction of ALS-associated Cu, Zn-superoxide dismutase aggregates in spinal cords of model mice

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However, as ChaC2 has a much lower activity to compare the enzymes accurately, 1 g of both ChaC1 and ChaC2 enzymes were incubated for 1.5 hours with varying concentrations of juglone