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Erythrocytes were lysed for 10 min using lysis buffer (BD Pharm Lyse, BD Bioscience, San Jose, CA, USA)

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In an autocrine mechanism, SASP factors reinforce the cellular senescence of the senescent cells themselves, while in a paracrine mechanism, they are responsible for inducing senescence in surrounding cells [35]

Cholecalciferol (D3) is metabolized to its active form, calcitriol, which increases the body's absorption of calcium and phosphorus

Neurobion contain vitamins b-complex (i.e B1, B6, B12) which provide numerous health benefits as it increases energy level, good for appetite, digestion, proper nerve function, and healthy brain function