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Briefly, to verify the targeted binding between miR-29b-3p and the GGCT gene, a 2000-bp DNA fragment (derived from the transcription start site of the GGCT gene) was inserted into the pmir-GLO-Basic vector to construct the wild-type pmir-GLO-GGCT-3 UTR reporter plasmid (wt) and the mutant pmir-GLO-GGCT-3 UTR reporter plasmid (mut)

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Nutrients, 17 (2), 284

Because of this and the fact that patients dont gain weight while on the medication, sitagliptin has become more popular as a second-line treatment for those with type 2 diabetes (Bennett RG, 2018)

35,37 The disease can widely affect multiple systems, including cardiovascular, nervous, and skeletal systems