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75 g of protein lysates were heated for 10 min at 95 C in Laemmli 6X SDS sample buffer (Boston BioProducts, # BP-111R) with 5% 2-mercaptoethanol (VWR Life Science #M131-100ml) and then ran on 420% Criterion TGX pre-cast gels (Bio-Rad #5671093)

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Unlicensed retail products do not become pharmacy products A commercial website selling vials without a patient prescription does not become a 503A pharmacy because PCAC voted yes

8.2, p 0.0 were considered to have GPX4 overexpression in 226 (45.1%) patients