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Annexin V/PI cell death assay U251 cells were treated with either 50 M lobeline and allowed to recover from hypoxia overnight, or with increasing concentrations (100400 M) and allowed to recover for 4 h
[35] Hydrophobicity scales, which rank amino acids from most hydrophobic to most hydrophilic, consistently place cysteine towards the hydrophobic end of the spectrum, even when they are based on methods that are not influenced by the tendency of cysteines to form disulfide bonds in proteins