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The present plasma-based metabolomic analysis was conducted using the same clinically characterized paediatric cohort previously reported in our salivary metabolomics study [24]

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Immunoblotting validation of the differentially expressed proteins The protein expression levels of NDUFS1, NDUFV2, SQSTM1, SDHA, and ERO1A were further analyzed by immunoblotting analysis

Women often notice the downstream effects years before connecting them to growth hormone: sleep becomes lighter, recovery from exercise takes longer, body fat shifts toward the abdomen, and skin begins losing density