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Response: We appreciate the commenters for providing clarification and delineating the steps that are necessary to prepare the room, equipment, and supplies

Reduced astrocytic glutamate uptake (via excitatory amino acid transporter 1/2 (EAAT1/2) downregulation) causes synaptic Glu accumulation, activating neuronal NMDA receptors and triggering calcium overload-dependent apoptosis and pyroptosis [274]

The pineal gland produces it, and it usually increases as night falls, peaks at midnight, and then progressively diminishes when dawn light starts to enter the retina

It activates VEGFR2 (vascular endothelial growth factor receptor 2), triggering the PI3K-Akt-eNOS cascade and elevating nitric oxide production

Lyophilized: Store at 20 C (4 F) in dry, dark conditions