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The implementation of the SetCys unit in a process enabling the modular and straightforward assembly of linear or backbone cyclized polypeptides is illustrated by the synthesis of biologically active cyclic hepatocyte growth factor variants

The Step-by-Step Protocol: How to Reconstitute MOTS-c Now we get to the main event

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The MitoCHAC1 nucleotide sequence was designed such that the initiating codon was replaced with the coding sequence for the first 25 amino acids of the ornithine transcarbamylase leader sequence 89 to facilitate targeting of exogenous CHAC1 to the mitochondrial matrix