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Electrolyte monitoring is not routinely required for all GLP-1 users, but becomes essential when patients experience persistent gastrointestinal symptoms or have additional risk factors such as chronic kidney disease, heart failure, age over 65, or concurrent diuretic use

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Library amplification was conducted using the NGS Tagment Index Kit for Illumina in a 50 L reaction containing 20 L of eluted DNA, 25 L of 2 Ultima Amplification Buffer, and 1 L each of 10 M P5 and P7 primers

This derivative exhibited relatively high inhibitory activity against ABTS and DPPH radicals (Tayade et al., 2022)