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Briefly, sodium bicarbonate, glucose, sodium pyruvate, and all 20 amino acids (except cystine and serine), biotin (B4639, Sigma), and vitamin B12 (V6629, Sigma) were added to the DMEM medium (#D9800-28, USBiological, Salem, MA) to match standard IMDM, supplement with 15% human AB serum and 15% dialyzed FBS (#SH300790340, Cytiva Hyclone), along with 10 ng/mL interleukin-3, 50 ng/mL stem cell factor and 2IU/mL erythropoietin

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Chem Soc Rev 39:329353 Carafoli E (2002) Calcium signaling: a tale for all seasons

[DOI] [PubMed] [Google Scholar] 60.Rudraiah S, Rohrer PR, Gurevich I, Goedken MJ, Rasmussen T, et al

Its involved in many body functions, including the formation of collagen, absorption of iron, the proper functioning of the immune system, wound healing, and the maintenance of cartilage, bones, and teeth