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Cell Culture and in vitro Model of Hemorrhagic Toxicity Astrocytes and 293T cells (Chinese Academy of Sciences, Shanghai) were cultured in dulbeccos modified eagle medium (DMEM) (Gibco, UA) added 10% fetal bovine serum (Gibco, Australia), following which they were incubated at 100% humidity in an environment containing 5% CO 2

This can be risky because an untreated B12 deficiency could lead to nerve issues down the line

Role and mechanism of gut microbiota in human disease

The result was clinically meaningful

Three-dimensional evaluation of the cytotoxicity and antibacterial properties of alpha lipoic acid-capped silver nanoparticle constructs for oral applications