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[DOI] [PMC free article] [PubMed] [Google Scholar] 58.Mulligan T., Frick M.F., Zuraw Q.C., Stemhagen A., McWhirter C

Do not refreeze after reconstitution , crystallization during freezing/thawing can damage the peptide structure

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If you wanna become a real HA-and-the-skin expert you can read way more about the topic at hyaluronic acid (including penetration-questions, differences between high and low molecular weight versions and a bunch of references to scientific literature)