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To exchange the linkers, maxiprepped libraries of M13-linked scFvs were amplified using Phusion Plus with a pool of overlapping primers annealing to either framework 4 (FW4) of the heavy chain or framework 1 (FW1) of the light chain

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A 2025 systematic review published in the Orthopaedic Journal of Sports Medicine examined 36 studies on BPC-157 published between 1993 and 2024