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Exe4 secretion in hepatocyte-derived cell lines A series of Exe secretion plasmids containing various SP and FCS (each 1 g) were transfected into Hepa1-6 and HepG2 cells in 12-well plates using Lipofectamine 3000 (Invitrogen) according to the manufactures protocol

Using either a mast cell stabilizer or a medication to reduce prostaglandin E2 synthesis (e.g., a COX2 inhibitor) stopped the hypersensitivity in the animal model of IBS.[ref] In the Lifehacks section (below), youll find a more comprehensive list of substances that also trigger mast cell degranulation