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Gestational age refers to the length of pregnancy, measured in weeks, from the first day of the last menstrual period (LMP) to the current date

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Finally, the membranes were incubated with a horseradish peroxidase (HRP)-conjugated antirabbit IgG secondary antibody (Santa Cruz, CA, USA), and bands were visualized using the Odyssey Fc Dual-Mode Imaging System (LI-COR Biosciences, Lincoln, NE, USA)