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HIV-1 Tat protein induces DNA damage in human peripheral blood B-lymphocytes via mitochondrial ROS production

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This supports the hypothesis that decreased wound contraction by myofibroblasts could contribute to delayed wound healing in smokers

In most cases, where the fusion protein is initially observed primarily in inclusion bodies, it is desirable to modify culture conditionssuch as lowering growth temperature, increasing aeration, or altering induction conditions toattempt to obtain soluble protein, rather than to solubilize inclusion bodies with denaturants and refold the protein after extraction