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This procedure ensured that animals were under deep anesthesia before euthanasia

TB500 Fragment (1723), also known as fequesetide, is a highpurity, research-grade heptapeptide derived from the active domain of thymosin beta4 (TB4), engineered to model actin regulation, cell migration, and tissue repair in controlled experimental systems

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The relevant independent Ethics Committee (Landesamt fr Gesundheit und Soziales Ethik-Kommision des Landes Berlin) reviewed and approved the protocol and the trial was conducted in accordance with Good Clinical Practice, the Declaration of Helsinki, and US FDA and European Medicines Agency guidelines for DDI studies [17, 18]