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To obtain serum, the blood was then allowed to clot for 10 minutes at room temperature before being centrifuged for 15 minutes at 3500 rpm using a SIGMA SM7000 centrifuge (SIGMA, UK)

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This proposed mechanism has been directly validated in animal models where the surgically severed vagus nerve attenuated anxiety- and depression-like behaviors in mice after oral antibiotic administration, providing direct evidence for neuropsychiatric effects induced by antibiotics through the gutbrain axis (Joo et al., 2023)

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Typically, a normal heart rates at rest should be: A heart rate of more than 100 beats per minute (BPM) is considered abnormally high heart rate in all age groups