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1 mM caffeine (Sigma, C1778 in PBS (pH 7.4) with 1% DMSO-d6) was used as a standard to quantify the ligand signals and determine the concentration of samples

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In plain terms, the stack hits weight loss from four directions: reduced appetite (GLP-1), better insulin signaling (GIP), more energy burn (glucagon), and stronger fullness (amylin)

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