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However, high agonist efficacy might result not only from a propensity to stabilize conformer 1, but also from an ability to promote G protein turnover, which could be hindered if conformer 1 were too long-lived

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Glucagon works by increasing energy expenditure ("I'm burning more fuel")

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CellTiter-Glo reagent was added to the wells according to the manufacturers instructions and luminescent signal was measured on a SpectraMax M3 microplate reader (Molecular Devices)