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To adjust for the possibility that the treatment reduced Buchnera numbers, we performed dPCR for a region of a control gene ( rne encoding ribonuclease 3)

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Briefly, plasma cleaned cover slips were incubated in 1M potassium hydroxide for 20 min, rinsed in deionised water and transferred to 1% (3-aminopropyl)trimethoxysilane diluted in methanol supplemented with 5% acetic acid for 20 min incubation