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To separate MCF derivatives from the reaction mixture, 400 L chloroform was added and vigorously mixed for 10 s followed by the addition of 400 L sodium bicarbonate (50 mM), and mixing for an additional 10 s

Many nutrients have their action INSIDE the cell, which means that testing serum and blood levels just give us an indication of what may be available but that is assuming everything else is working properly: cellular activity, enzyme activity, receptor activity, etc

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