Cells were then labelled using SNAP-Surface-549 (1 M) in complete medium for 30 minutes on ice to label surface receptor (contributed to by a recycled receptor population and a further population that were not internalised during the agonist stimulation step), fixed with 4% paraformaldehyde, and mounted in Diamond Prolong mounting medium
Uguen K, Ka C, Collod-Broud G, Le Tertre M, Guellec J, Frec C, Broud C, Callebaut I, Le Gac G (2023) The spectra of disease-causing mutations in the ferroportin 1 (SLC40A1) encoding gene and related iron overload phenotypes (hemochromatosis type 4 and ferroportin disease)
Instead, the modern gold standard is defined by how effectively we can communicate with our cells
We also examined the efficacy of 4 other clinically-approved drugs that functionally behave as either OXPHOS inhibitors (Irinotecan, Sorafenib, Niclosamide) or glycolysis inhibitors (Stiripentol) (Figure 11)
Ascites has many causes, and it is important to differentiate ascites secondary to portal hypertension from ascites due to other causes
