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After sample denaturation and electrophoresis, membranes were blocked with 5% milk in TBS-T (120 mM Tris-HCl, pH 7.4, 150 mM NaCl, and 0.05% Tween 20) for 1 h at room temperature

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A peptides certificate of analysis should be independently verifiable meaning it comes from a third-party lab rather than the suppliers in-house testing alone since that independence is what gives the purity claim scientific credibility