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Individual GST protein spots observed on 2D SDS PAGE were detected and quantified using Progenesis PG220, software version 2006 (Nonlinear Dynamics Ltd.), as described previously (Morphew et al ., Reference Morphew, MacKintosh, Hart, Prescott, LaCourse and Brophy2014;, Reference Morphew, Wilkinson, Mackintosh, Jahndel, Paterson, McVeigh, Abbas Abidi, Saifullah, Raman, Ravikumar and LaCourse2016)