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We challenged these results from cathepsin D activity-based probes by assaying proteolytic cleavage of DQ-BSA, a quenched fluorophore coupled to BSA taken up by endocytosis that emits fluorescence upon lysosomal proteolysis

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Subsequently, we generated Gadd45a knockout mice and found that these mice exhibit significant deficits in social ability, as well as autistic-like phenotypes such as increased digging behaviors