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The Obesity Collective is strongly supportive of actions to reduce weight stigma and firmly believes that blaming individuals doesnt work, is counterproductive, has prevented the initiation of effective therapies and has distracted us from a broader understanding of the drivers of obesity and strategies to address them

RNA isolation and qRT-PCR Total RNA was extracted from primary astrocytes and striatum tissue using TRIzol reagent (Invitrogen, 15596026) and reversed transcribed into cDNA using PrimeScript RT Master Mix (Takara, RR036A)

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