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After incubating, the blots were rinsed 3 times using TBST for 5 min, incubated for one hour at room temperature with the matching secondary antibody solution conjugated with horseradish peroxidase, and then rinsed with TBST once more

2021;5:A167

Formulations compounded at pH 7.4 (physiological pH) generally demonstrate superior stability compared to those at lower pH values, all else being equal