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We screened candidate mutant lines by PCR and Sanger sequencing and identified the Atg8a 12 allele that carries an 8-bp deletion based on sequencing data (Fig S2A), which causes frameshift in all isoforms of the Atg8a gene
References Witschi A, Reddy S, Stofer B, Lauterburg BH
Gut microbiota influence acute pancreatitis through inflammatory proteins: a Mendelian randomization analysis
Dark circles have two primary causes that are frequently conflated but require different active responses: vascular pooling in the blood vessels around the eye, which produces a bluish-purple discolouration most visible through thin skin in that area, and surface pigmentation from UV exposure, post-inflammatory marks, or accumulated melanin, which produces a brownish discolouration
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