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2D projection analysis of GPCR complexes by negative stain electron microscopy
Upper parts of the nitrocellulose membranes we incubated with an anti-calsequestrin antibody (#ab3516, abcam, Cambridge, England) and the remainder of the nitrocellulose membrane was incubated with an antibody against phospholamban phosphorylated at serine 16 or against total phospholamban (#A010-12 AP, A1, Badrilla, Leeds, England) or agonist phosphorylated TnI, as reported (e.g
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