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Kiefer HL, Hanley TM, Marcello JE, Karthik AG, Viglianti GA

Generation of closed SpyLocks SpyLock constructs (including BiLockCatcher fusions) were incubated in PBS/2.5 mM TCEP for one hour, followed by buffer-exchange (MiniTrap G-25 columns, Cytiva) into DTNB-labeling buffer (100 mM sodium phosphate, 1 mM EDTA, pH 8.0) or biotin-labeling buffer (PBS pH 7.4, 1 mM EDTA)