Apoptosis assay (flow cytometry) The apoptosis rate of Raji cells was determined by flow cytometry using a dead cell apoptosis kit with Annexin V Alexa Fluor 488 and propidium iodide (Invitrogen)
RT-qPCR analyses validated the upregulation of ATF4 target genes, including SLC1A1, SLC7A8 , phosphoserine aminotransferase 1 ( PSAT1 ), and serine hydroxymethyltransferase 2 ( SHMT2 ) in T315I-Bcr-Abl cells (Fig
The richest sources of Vitamin B12 include: meat (particularly organ meats such as liver), fish, milk, dairy, and eggs
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How to prevent it: Start at 2 to 3 times per week for the first 2 weeks