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Tirzepatide has since become a foundational reference compound in incretin pharmacology, with hundreds of peer-reviewed publications characterising its receptor binding kinetics, biased signalling profile, and downstream effects across pancreatic, hepatic, adipose, and central nervous system tissues

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RNA sequencing Sequencing libraries were generated using the NEBNext UltraTM RNA Library Prep Kit for Illumina following the manufacturers recommendations, and index codes were added to attribute sequences to each sample