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Western blot assay Cells (1 10 6 ) were grown to 7080% confluency and treated with DMSO control, (1S,3R)-RSL3 (RSL3, Catalog # 19,288, Cayman Chemical, MI) or Fer1 (Medchemexpress, NJ) as per the experiments

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Similarly, endothelium-mediated vasodilator responses were restored when a transgene for GPx1 was introduced into the CBS +/ mice by crossbreeding transgenic GPx1 overexpressing mice and CBS deficient mice, suggesting that increased expression of GPx1 could compensate for the negative effects of homocysteine on bioactive NO [60]

Amino acid metabolism in lupus