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The suspended liver microsomes were placed on ice in prechilled microcentrifuge tubes, allowing for the addition of 640 ul of 0.1 M Tris buffer and 10 ul of 500 uM test compound to the sample

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Microchim Acta 183(4):14091416 Chang CW, Tseng WL (2010) Gold nanoparticle extraction followed by capillary electrophoresis to determine the total, free, and protein-bound aminothiols in plasma

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If youre closer to your next scheduled dose, skip the missed dose and resume your normal scheduledo not double up