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doi: 10.1016/j.molcel.2020.11.024 64 LiuJKangRTangD
I lost about 170 pounds getting here, a lot of it on tirzepatide, and that is its own long story (told in full here)
American journal of kidney diseases [Available on 1st August 1998] [Accessed on 19th July 2022] 3
4.Enzyme marker inactivation or substrate failureMix the enzyme conjugate and substrate and check and judge by rapid color development Problem description: Low readingsPossible causes and corresponding countermeasures: 1.The microplate reader is not set up correctlyCheck wavelength and filter settings on the microplate reader/Turn on the ELISA reader in advance to preheat Problem description: Large coefficient of variationPossible causes and corresponding countermeasures: 1.Incorrect addition of liquidCheck the filling status Problem description: High background valuePossible causes and corresponding countermeasures: 1.The working concentration of the detection antibody is too highUse the recommended dilution factor 2.Incomplete washing of ELISA plateEnsure that each step is cleaned completely
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