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Furthermore, the strong negative charge on the noncanonical S -sulfonate, as well as the presence of arginine residues R370 and R413 in the Rhod active site, suggests that the interaction between the PDO and Rhod domain is facilitated by electrostatic steering of the loop

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Preclinical research examining MT-1 and related melanocortin agonists has employed variable dosing protocols depending on study design, research endpoint, and experimental model