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Sperm samples were centrifuged through a PureSperm 80/40 (Nidacon, Gothenburg, Sweden) gradient at at 1,500 rpm for 8 min

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Neurogenesis in males has a different trajectory compared with females The present study found that males, but not females, showed substantial changes in the density of BrdU-ir cells across time points with an early increase from 24 h to one week followed by a substantial decrease from one to two weeks

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Previous research has shown that substance P is highly expressed in the heart