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Proteins were transferred to PVDF membranes (Immobilon-P, 0.45-m pore size, Sigma-Aldrich) using a wet transfer Western blot system (Bio-Rad) and membranes blocked in 5% BSA in 1x TBS/Tween (20 mM Tris-HCl, 150 mM NaCl, 0.1% Tween-20) before incubation with a mouse monoclonal anti-pan-phospho-serine primary antibody (M380B, Biolegend, 1:1,000) also in 5% BSA in 1x TBS/Tween followed by 3x washes in 1x TBS/Tween and incubation with goat anti-mouse HRP secondary antibody (ab205719, Abcam, 1:5,000) before being developed using the Clarity Western enhanced chemiluminescence substrate system (Bio-Rad) in a ChemiDoc imaging system (Bio-Rad)

Oncogene 26: 11011109