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Additionally, GGCT knockdown concomitantly reduced 5- oxoproline (5-OP) levels in MHCC97H cells, consistent with 5-OP being the direct enzymatic product of GGCT activity, and exogenous 5-OP rescued proliferation under Gln-deprived conditions (Fig

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Representative tissue specimens were embedded in paraffin, sectioned at 4 m, stained with hematoxylin and eosin (H&E), and evaluated by light microscopy using an Olympus 71 digital camera and an Olympus BX51 microscope (Japan) acquiring digital images saved as uncompressed 24-bit RGB TIFF files

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