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LC-GFP or LC-SYNCRIP plasmids were transfected into cells using Lipofectamine 3000 (L3000015, Thermo Fisher Scientific, USA) according to the manufacturer's instructions to generate control or SYNCRIP-deficient (KO) cells

Risk factors for B12 deficiency include strict vegetarian diets, gastrointestinal conditions, and certain medications

Funding This study was supported by the State Key Laboratory of Trauma, Burn and Combined Injury (SKLYQ202002 to YC), Southwest Hospital (SWH2018BJKJ-05 to YC), National Natural Science Foundation of China (82030036 to HF), and Chongqing Talent Program (4139Z2391 to HF)

However, like any physical activity, it can sometimes lead to unwanted consequences, such as Golfers Elbow

Seek medical attention immediately, infections require antibiotic treatment