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Overexpressed proteins were purified on a Ni-NTA column (Cytiva) with an elution buffer containing 50 mM NaH 2 PO 4 , 300 mM NaCl, and 300 mM imidazole

Patients who should not receive BPC-157 include: those with active or recent malignancies (due to angiogenic concerns), pregnant or breastfeeding women (no reproductive toxicology data in humans), patients on anticoagulation therapy (theoretical bleeding risk from angiogenesis at ulcer sites), and minors (no pediatric data of any kind)

Beyond treatment, reclaimed water distribution presents persistent logistical challenges