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Ratiometric time-course measurements were carried out with initially oxidized or reduced roGFP2 on a BMG POLARstar Omega fluorescence plate reader with a filter-based excitation at 39010 and 48010 nm

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These treatments also increased the expression of genes involved in fatty acid, amino acid, and inorganic solute transport, as well as cell junction proteins, but not genes associated with protein digestion ( TMPRSS15 and ANPEP ) (Supplementary Fig

In addition, loss of podocytes, either due to apoptosis and/or podocyte detachment from the glomerular basement membrane, was shown to be an early event in the development of diabetic nephropathy in humans and various animal models of diabetic nephropathy [196,197,198]