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One group developed a drug-eluting hydrogel system which, when implanted in a surgical resection site, has the benefits of delivering high doses of the drug directly to the target site, reducing systemic side effects, with continuous and sustained release (unlike intermittent doses with other drug delivery methods) ( As well as incorporating growth factors and drug compounds into biomaterials, more recent studies are beginning to introduce other compounds such as antibiotics and antioxidants (Kim et al., 2004

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After sonication, cell lysates were placed on ice for 30 min and centrifugated at 14,000 rpm for 10 min, and the supernatant was then transferred to a new EP tube