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KG-dependent dioxygenases CRISPR library construction We constructed a pooled sgRNA library containing 64 sgRNAs targeting various genes whose enzymes require KG as a co-factor for their activity in addition to controls targeting intragenic regions as described previously (Joung et al., 2017) ( Table S1 )

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BBA-Gen Subjects 1830:33043316 Gogos A, Shapiro L (2002) Large conformational changes in the catalytic cycle of glutathione synthase

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Amini A, Correa AM, Komaki R, Chang JY, Tsao AS, Roth JA, Swisher SG , Rice DC, Vaporciyan AA, Lin SH