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The membrane-bound complex was solubilized by 0.5% (w/v) lauryl maltose neopentyl glycol (LMNG) supplemented with 0.03% (w/v) cholesteryl hemisuccinate (CHS) for 2 h at 4 C in the presence of 10 M semaglutide, 10 g mL 1 Nb35, and 25 mU mL 1 apyrase

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Perspect Biol Med 41(3):359390