To generate the plasmid for the coexpression of the H63F-mutated tyrosinase and caddie, the region containing the T7 promoter and mutated tyrC gene was amplified with the forward primer 5-GCACGCATGCGAAATTAATACGACTCAC-3 (the underline indicates the SphI site) and the reverse primer 5-CTATGCATGCCAAAAAACCCCTCAAGAC-3 (the underline indicates the SphI site) by using the mutated plasmid as a template
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doi: 10.1038/s42255-023-00966-w
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STEP 4 Move the WHITE patch from the 3 oclock position to the 6 oclock position
