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The protein was mixed in a 1:1 ratio with droplet formation buffer (50 mM Tris pH 7.5, 125 mM NaCl, 10% glycerol, 1 mM DTT, with 20% or 40% PEG8000) and 2 l of the suspension was loaded onto a hand-made cassette with a clean glass slide, spacers, and a coverslip

European Journal of Clinical Nutrition , 69 (8), 969971

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