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Initially, the alterations in intracellular protein concentrations post-treatment were assessed, and the principal component analysis (PCA) outcomes demonstrated a notable divergence in protein expression between the D-CuP-treated group and the PBS-treated control group (Fig

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Mass spectrometry identity confirmation: Molecular weight must be verified via LC-MS to confirm the peptide sequence matches the expected 15-amino acid structure and the confirmed molecular weight of approximately 1,419 Daltons